[2018-12-04 13:43:33] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-12-04 13:43:33] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-12-04 13:43:33] Checking for Bowtie index files (genome).. [2018-12-04 13:43:33] Checking for reference FASTA file [2018-12-04 13:43:33] Generating SAM header for Bowtie2Index/genome [2018-12-04 13:43:38] Reading known junctions from GTF file [2018-12-04 13:43:44] Preparing reads left reads: min. length=50, max. length=50, 46541849 kept reads (27502 discarded) right reads: min. length=50, max. length=50, 46475319 kept reads (94032 discarded) [2018-12-04 14:04:57] Building transcriptome data files /scratch/9977263.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-12-04 14:05:16] Building Bowtie index from RefSeq_GeneBody.fa [2018-12-04 14:14:42] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-12-04 14:38:53] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-12-04 15:01:43] Resuming TopHat pipeline with unmapped reads [2018-12-04 15:01:44] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-12-04 15:11:03] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2018-12-04 15:13:14] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2018-12-04 15:14:32] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-12-04 15:24:11] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2018-12-04 15:26:22] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2018-12-04 15:28:26] Searching for junctions via segment mapping [2018-12-04 15:33:20] Retrieving sequences for splices [2018-12-04 15:35:59] Indexing splices [2018-12-04 15:36:25] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2018-12-04 15:36:48] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2018-12-04 15:37:09] Joining segment hits [2018-12-04 15:40:12] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2018-12-04 15:40:39] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2018-12-04 15:41:07] Joining segment hits [2018-12-04 15:44:20] Reporting output tracks ----------------------------------------------- [2018-12-04 16:22:27] A summary of the alignment counts can be found in /scratch/9977263.1.linga/tophat2/align_summary.txt [2018-12-04 16:22:27] Run complete: 02:38:54 elapsed