-----------------------
Start of variable list:
-----------------------
Sample_ID:
G170_M28
Dataset_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G170
Sample_Labels_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G170/Scripts/00_Setup_Pipeline
STRAND_RULE:
1+-,1-+,2++,2--
BU_User:
kkarri
VM_DIR_UCSC:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G170
SCRIPT_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G170/Scripts/11_UCSC_BigWig
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Mon Sep 16 11:18:39 EDT 2019
Running on node : scc-kb1
Current directory : /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G170/Scripts/11_UCSC_BigWig
Current job ID : 9374133
Current job name : Step_11_G170_M28
Task index number : undefined
Parameter for multiple cores : 1
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/9374133.1.linga

Loading required modules...


INPUT_BAM:
G170_M28_primary_unique.bam


List files in scratch directory:

total 2.1G
drwx------   4 kkarri waxmanlab 4.0K Sep 16 11:26 .
drwxrwxrwt. 48 root   root      4.0K Sep 16 11:18 ..
-rw-r--r--   1 kkarri waxmanlab 2.1G Sep 16 11:26 G170_M28_primary_unique.bam
drwxr-xr-x   2 kkarri waxmanlab 4.0K Sep 16 11:26 UCSC_BigWig
drwxr-xr-x   2 kkarri waxmanlab 4.0K Sep 16 11:26 UCSC_Tools
-rwxr-xr-x   1 kkarri waxmanlab  584 Sep 16 11:26 mm9.chrom.sizes

Starting to run my commands


Starting samtools sort and index


Finished samtools sort and index


Starting bam2wig.py command

Printing command:
bam2wig.py --input-file=G170_M28_sorted.bam --chromSize=mm9.chrom.sizes --out-prefix=G170_M28 --wigsum=1000000000 --strand=1+-,1-+,2++,2--

Skip multi-hits:False

Finished bam2wig.py command


Sample from wiggle files:

==> G170_M28.Forward.wig <==
variableStep chrom=chrY
1454145	0.15
1454146	0.15
1454147	0.15
1454148	0.15
1454149	0.15
1454150	0.15
1454151	0.15
1454152	0.15
1454153	0.15

==> G170_M28.Reverse.wig <==
variableStep chrom=chrY
322938	-0.15
322939	-0.15
322940	-0.15
322941	-0.15
322942	-0.15
322943	-0.15
322944	-0.15
322945	-0.15
322946	-0.15

#--------------------------------
G170_M28.Forward.wig
#--------------------------------
Line count where 2nd column does not equal "-":
102123497
Line count where 2nd column is only a negative sign:
0
Copy G170_M28.Forward.wig to UCSC_Tools folder
Copy mm9.chrom.sizes to UCSC_Tools folder
Start wigToBigWig
End wigToBigWig

#--------------------------------
G170_M28.Reverse.wig
#--------------------------------
Line count where 2nd column does not equal "-":
98403696
Line count where 2nd column is only a negative sign:
0
Copy G170_M28.Reverse.wig to UCSC_Tools folder
Copy mm9.chrom.sizes to UCSC_Tools folder
Start wigToBigWig
End wigToBigWig

List of files in UCSC_Tools folder:

-rw-r--r-- 1 kkarri waxmanlab 1464543839 Sep 16 13:16 ./UCSC_Tools/G170_M28.Forward.wig
-rw-r--r-- 1 kkarri waxmanlab 1510726453 Sep 16 13:20 ./UCSC_Tools/G170_M28.Reverse.wig
-rwxr-xr-x 1 kkarri waxmanlab        584 Sep 16 13:20 ./UCSC_Tools/mm9.chrom.sizes
-rwx------ 1 kkarri waxmanlab    1012225 Sep 16 11:26 ./UCSC_Tools/wigToBigWig

Copy output to storage dir


List files in scratch

total 6.9G
drwx------   4 kkarri waxmanlab 4.0K Sep 16 11:48 .
drwxrwxrwt. 47 root   root      4.0K Sep 16 13:20 ..
-rw-r--r--   1 kkarri waxmanlab 1.4G Sep 16 13:14 G170_M28.Forward.wig
-rw-r--r--   1 kkarri waxmanlab 1.5G Sep 16 13:14 G170_M28.Reverse.wig
-rw-r--r--   1 kkarri waxmanlab 2.1G Sep 16 11:26 G170_M28_primary_unique.bam
-rw-r--r--   1 kkarri waxmanlab 2.1G Sep 16 11:41 G170_M28_sorted.bam
-rw-r--r--   1 kkarri waxmanlab 3.8M Sep 16 11:42 G170_M28_sorted.bam.bai
drwxr-xr-x   2 kkarri waxmanlab 4.0K Sep 16 13:21 UCSC_BigWig
drwxr-xr-x   2 kkarri waxmanlab 4.0K Sep 16 13:20 UCSC_Tools
-rwxr-xr-x   1 kkarri waxmanlab  584 Sep 16 11:26 mm9.chrom.sizes
==========================================================
Finished on : Mon Sep 16 13:23:18 EDT 2019
2 hours, 4 minutes and 39 seconds elapsed.
==========================================================