[2020-03-09 05:49:10] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2020-03-09 05:49:10] Checking for Bowtie Bowtie version: 2.3.4.1 [2020-03-09 05:49:11] Checking for Bowtie index files (genome).. [2020-03-09 05:49:11] Checking for reference FASTA file [2020-03-09 05:49:11] Generating SAM header for Bowtie2Index/genome [2020-03-09 05:49:18] Reading known junctions from GTF file [2020-03-09 05:49:22] Preparing reads left reads: min. length=75, max. length=75, 30315875 kept reads (17126 discarded) [2020-03-09 06:00:40] Building transcriptome data files /scratch/3763306.1.linga/tophat2/tmp/RefSeq_GeneBody [2020-03-09 06:01:01] Building Bowtie index from RefSeq_GeneBody.fa [2020-03-09 06:09:08] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2020-03-09 06:22:39] Resuming TopHat pipeline with unmapped reads [2020-03-09 06:22:40] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2020-03-09 06:45:23] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/3) [2020-03-09 06:47:46] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/3) [2020-03-09 06:50:03] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/3) [2020-03-09 06:52:17] Searching for junctions via segment mapping [2020-03-09 06:57:04] Retrieving sequences for splices [2020-03-09 06:59:36] Indexing splices [2020-03-09 07:00:00] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/3) [2020-03-09 07:00:35] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/3) [2020-03-09 07:01:06] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/3) [2020-03-09 07:01:33] Joining segment hits [2020-03-09 07:05:15] Reporting output tracks ----------------------------------------------- [2020-03-09 07:35:02] A summary of the alignment counts can be found in /scratch/3763306.1.linga/tophat2/align_summary.txt [2020-03-09 07:35:02] Run complete: 01:45:51 elapsed