[2020-03-09 05:48:40] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2020-03-09 05:48:40] Checking for Bowtie Bowtie version: 2.3.4.1 [2020-03-09 05:48:41] Checking for Bowtie index files (genome).. [2020-03-09 05:48:41] Checking for reference FASTA file [2020-03-09 05:48:41] Generating SAM header for Bowtie2Index/genome [2020-03-09 05:48:46] Reading known junctions from GTF file [2020-03-09 05:48:50] Preparing reads left reads: min. length=75, max. length=75, 27012546 kept reads (15600 discarded) [2020-03-09 05:58:36] Building transcriptome data files /scratch/3763305.1.linga/tophat2/tmp/RefSeq_GeneBody [2020-03-09 05:58:57] Building Bowtie index from RefSeq_GeneBody.fa [2020-03-09 06:06:18] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2020-03-09 06:16:15] Resuming TopHat pipeline with unmapped reads [2020-03-09 06:16:15] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2020-03-09 06:34:05] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/3) [2020-03-09 06:36:08] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/3) [2020-03-09 06:38:12] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/3) [2020-03-09 06:40:38] Searching for junctions via segment mapping [2020-03-09 06:44:33] Retrieving sequences for splices [2020-03-09 06:46:43] Indexing splices [2020-03-09 06:47:04] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/3) [2020-03-09 06:47:32] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/3) [2020-03-09 06:47:59] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/3) [2020-03-09 06:48:23] Joining segment hits [2020-03-09 06:51:36] Reporting output tracks ----------------------------------------------- [2020-03-09 07:12:49] A summary of the alignment counts can be found in /scratch/3763305.1.linga/tophat2/align_summary.txt [2020-03-09 07:12:49] Run complete: 01:24:08 elapsed