FastQCFastQC Report
Sun 8 Mar 2020
SRR7721158.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7721158.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27028146
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG14148755.234820767950565TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGG1022170.3781872422917946TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG958530.35464141713604774TruSeq Adapter, Index 10 (100% over 63bp)
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAA894610.3309919962693704No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCGTCTGCTTGAAAAAAAAAAGG504110.18651297798968527TruSeq Adapter, Index 10 (98% over 63bp)
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAAC495770.18342730574268765No Hit
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGTCGAGAAGCAGGTGTCCTTGTCAGCA473060.17502495361687034No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG457430.169242093038864TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGGCTTCTGCTTGAAAAAAAAAAGG431750.15974088640782094TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG388320.14367245167315582TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTATGCTTGAAAAAAAAAAGG367670.1360322679920406TruSeq Adapter, Index 10 (98% over 63bp)
CTGCGTACGTCGGGAGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGA346680.12826628951908134No Hit
GTCTTCTCATGCAGCAGACACACACGGTTCAGGATTGCAGACAGATAGTCTTCCACACAAGGCAGTCTCTGATCT344470.12744862337209514No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATT329200.12179895727957071No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCC322400.11928306144269014No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCCGGGAAGGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGA316880.11724074599863417No Hit
GGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGA311790.11535752396779268No Hit
GGAGGATTGGCTTCAGCGCAGCACTTTTCCAGAGTGGCTTCATATTTCTTAGCAAGTCTCAGCAACAGGGATACA301810.11166507684248857No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTA282230.10442077677100013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)