FastQCFastQC Report
Sun 8 Mar 2020
SRR7721172.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7721172.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27193868
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGTCGAGAAGCAGGTGTCCTTGTCAGCA567330.20862423837609273No Hit
CTCTTGTTTTGTACAGCAGTCAGCCAGTTCACCATAGTTTTCACGGAGGTTTGGAATGGCACACAACTTATCTCC428960.15774144376960278No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG412320.15162241723023734RNA PCR Primer, Index 38 (100% over 43bp)
CTTGTTTTGTACAGCAGTCAGCCAGTTCACCATAGTTTTCACGGAGGTTTGGAATGGCACACAACTTATCTCCAA391360.14391479726238282No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAAT361470.13292334874906359No Hit
CTTCCGTACGCCACATTTCCCACGCCGCGACGCGCGGCGGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCC294660.10835531010152731No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCATAGTGGGGTATCTAATCCCAGTTTGG279960.10294967968514078No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)