FastQCFastQC Report
Sun 8 Mar 2020
SRR8186305.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8186305.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24672546
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGTCGAGAAGCAGGTGTCCTTGTCAGCA677680.2746696672487712No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG390320.1582001306229199RNA PCR Primer, Index 44 (100% over 43bp)
CTTGTTTTGTACAGCAGTCAGCCAGTTCACCATAGTTTTCACGGAGGTTTGGAATGGCACACAACTTATCTCCAA332690.13484218450742783No Hit
CTTCCGTACGCCACATTTCCCACGCCGCGACGCGCGGCGGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCC316280.12819106710754538No Hit
CTGCGTACGTCGGGAGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGA316220.12816674857957505No Hit
CTCTTGTTTTGTACAGCAGTCAGCCAGTTCACCATAGTTTTCACGGAGGTTTGGAATGGCACACAACTTATCTCC294640.11942018468625004No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATA259790.10529517302348936No Hit
GGAGGATTGGCTTCAGCGCAGCACTTTTCCAGAGTGGCTTCATATTTCTTAGCAAGTCTCAGCAACAGGGATACA247760.10041930816544024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)