[2020-03-09 05:48:26] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2020-03-09 05:48:26] Checking for Bowtie Bowtie version: 2.3.4.1 [2020-03-09 05:48:27] Checking for Bowtie index files (genome).. [2020-03-09 05:48:27] Checking for reference FASTA file [2020-03-09 05:48:27] Generating SAM header for Bowtie2Index/genome [2020-03-09 05:48:28] Reading known junctions from GTF file [2020-03-09 05:48:30] Preparing reads left reads: min. length=75, max. length=75, 24067329 kept reads (10033 discarded) [2020-03-09 05:53:18] Building transcriptome data files /scratch/3763317.1.c/tophat2/tmp/RefSeq_GeneBody [2020-03-09 05:53:27] Building Bowtie index from RefSeq_GeneBody.fa [2020-03-09 05:57:57] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2020-03-09 06:07:40] Resuming TopHat pipeline with unmapped reads [2020-03-09 06:07:40] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2020-03-09 06:13:03] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/3) [2020-03-09 06:13:48] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/3) [2020-03-09 06:14:30] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/3) [2020-03-09 06:15:06] Searching for junctions via segment mapping [2020-03-09 06:18:26] Retrieving sequences for splices [2020-03-09 06:19:31] Indexing splices [2020-03-09 06:19:43] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/3) [2020-03-09 06:20:06] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/3) [2020-03-09 06:20:25] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/3) [2020-03-09 06:20:38] Joining segment hits [2020-03-09 06:22:07] Reporting output tracks ----------------------------------------------- [2020-03-09 06:44:56] A summary of the alignment counts can be found in /scratch/3763317.1.c/tophat2/align_summary.txt [2020-03-09 06:44:56] Run complete: 00:56:29 elapsed