[2020-03-09 07:03:54] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2020-03-09 07:03:54] Checking for Bowtie Bowtie version: 2.3.4.1 [2020-03-09 07:03:55] Checking for Bowtie index files (genome).. [2020-03-09 07:03:55] Checking for reference FASTA file [2020-03-09 07:03:55] Generating SAM header for Bowtie2Index/genome [2020-03-09 07:03:56] Reading known junctions from GTF file [2020-03-09 07:03:58] Preparing reads left reads: min. length=75, max. length=75, 12884230 kept reads (5767 discarded) [2020-03-09 07:06:30] Building transcriptome data files /scratch/3763323.1.c/tophat2/tmp/RefSeq_GeneBody [2020-03-09 07:06:39] Building Bowtie index from RefSeq_GeneBody.fa [2020-03-09 07:11:04] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2020-03-09 07:15:11] Resuming TopHat pipeline with unmapped reads [2020-03-09 07:15:11] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2020-03-09 07:18:40] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/3) [2020-03-09 07:19:07] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/3) [2020-03-09 07:19:33] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/3) [2020-03-09 07:19:55] Searching for junctions via segment mapping [2020-03-09 07:21:47] Retrieving sequences for splices [2020-03-09 07:22:52] Indexing splices [2020-03-09 07:23:03] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/3) [2020-03-09 07:23:12] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/3) [2020-03-09 07:23:20] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/3) [2020-03-09 07:23:26] Joining segment hits [2020-03-09 07:24:47] Reporting output tracks ----------------------------------------------- [2020-03-09 07:40:30] A summary of the alignment counts can be found in /scratch/3763323.1.c/tophat2/align_summary.txt [2020-03-09 07:40:30] Run complete: 00:36:36 elapsed