[2020-03-09 07:38:12] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2020-03-09 07:38:12] Checking for Bowtie Bowtie version: 2.3.4.1 [2020-03-09 07:38:15] Checking for Bowtie index files (genome).. [2020-03-09 07:38:15] Checking for reference FASTA file [2020-03-09 07:38:15] Generating SAM header for Bowtie2Index/genome [2020-03-09 07:38:19] Reading known junctions from GTF file [2020-03-09 07:38:23] Preparing reads left reads: min. length=75, max. length=75, 50343089 kept reads (21317 discarded) [2020-03-09 07:55:21] Building transcriptome data files /scratch/3763324.1.linga/tophat2/tmp/RefSeq_GeneBody [2020-03-09 07:55:40] Building Bowtie index from RefSeq_GeneBody.fa [2020-03-09 08:02:38] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2020-03-09 08:22:15] Resuming TopHat pipeline with unmapped reads [2020-03-09 08:22:15] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2020-03-09 08:46:23] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/3) [2020-03-09 08:49:28] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/3) [2020-03-09 08:52:30] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/3) [2020-03-09 08:54:59] Searching for junctions via segment mapping [2020-03-09 08:59:46] Retrieving sequences for splices [2020-03-09 09:01:55] Indexing splices [2020-03-09 09:02:25] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/3) [2020-03-09 09:03:00] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/3) [2020-03-09 09:03:44] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/3) [2020-03-09 09:04:08] Joining segment hits [2020-03-09 09:08:26] Reporting output tracks ----------------------------------------------- [2020-03-09 09:47:28] A summary of the alignment counts can be found in /scratch/3763324.1.linga/tophat2/align_summary.txt [2020-03-09 09:47:28] Run complete: 02:09:15 elapsed