[2020-03-09 05:49:02] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2020-03-09 05:49:02] Checking for Bowtie Bowtie version: 2.3.4.1 [2020-03-09 05:49:02] Checking for Bowtie index files (genome).. [2020-03-09 05:49:02] Checking for reference FASTA file [2020-03-09 05:49:02] Generating SAM header for Bowtie2Index/genome [2020-03-09 05:49:07] Reading known junctions from GTF file [2020-03-09 05:49:13] Preparing reads left reads: min. length=75, max. length=75, 31011620 kept reads (19046 discarded) [2020-03-09 06:00:26] Building transcriptome data files /scratch/3763310.1.linga/tophat2/tmp/RefSeq_GeneBody [2020-03-09 06:00:46] Building Bowtie index from RefSeq_GeneBody.fa [2020-03-09 06:08:20] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2020-03-09 06:19:19] Resuming TopHat pipeline with unmapped reads [2020-03-09 06:19:19] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2020-03-09 06:45:40] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/3) [2020-03-09 06:48:53] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/3) [2020-03-09 06:51:57] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/3) [2020-03-09 06:55:31] Searching for junctions via segment mapping [2020-03-09 06:59:32] Retrieving sequences for splices [2020-03-09 07:01:44] Indexing splices [2020-03-09 07:02:06] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/3) [2020-03-09 07:02:41] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/3) [2020-03-09 07:03:14] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/3) [2020-03-09 07:03:47] Joining segment hits [2020-03-09 07:07:11] Reporting output tracks ----------------------------------------------- [2020-03-09 07:31:11] A summary of the alignment counts can be found in /scratch/3763310.1.linga/tophat2/align_summary.txt [2020-03-09 07:31:11] Run complete: 01:42:09 elapsed