[2020-03-09 05:48:27] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2020-03-09 05:48:27] Checking for Bowtie Bowtie version: 2.3.4.1 [2020-03-09 05:48:27] Checking for Bowtie index files (genome).. [2020-03-09 05:48:27] Checking for reference FASTA file [2020-03-09 05:48:27] Generating SAM header for Bowtie2Index/genome [2020-03-09 05:48:34] Reading known junctions from GTF file [2020-03-09 05:48:38] Preparing reads left reads: min. length=75, max. length=75, 27162282 kept reads (14633 discarded) [2020-03-09 06:00:08] Building transcriptome data files /scratch/3763313.1.linga/tophat2/tmp/RefSeq_GeneBody [2020-03-09 06:00:27] Building Bowtie index from RefSeq_GeneBody.fa [2020-03-09 06:08:16] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2020-03-09 06:19:09] Resuming TopHat pipeline with unmapped reads [2020-03-09 06:19:10] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2020-03-09 06:36:08] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/3) [2020-03-09 06:38:40] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/3) [2020-03-09 06:40:52] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/3) [2020-03-09 06:42:46] Searching for junctions via segment mapping [2020-03-09 06:47:11] Retrieving sequences for splices [2020-03-09 06:49:42] Indexing splices [2020-03-09 06:50:11] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/3) [2020-03-09 06:50:36] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/3) [2020-03-09 06:50:58] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/3) [2020-03-09 06:51:16] Joining segment hits [2020-03-09 06:54:56] Reporting output tracks ----------------------------------------------- [2020-03-09 07:19:02] A summary of the alignment counts can be found in /scratch/3763313.1.linga/tophat2/align_summary.txt [2020-03-09 07:19:02] Run complete: 01:30:34 elapsed