[2019-04-30 23:03:24] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2019-04-30 23:03:24] Checking for Bowtie Bowtie version: 2.2.4.0 [2019-04-30 23:03:25] Checking for Bowtie index files (genome).. [2019-04-30 23:03:25] Checking for reference FASTA file [2019-04-30 23:03:25] Generating SAM header for Bowtie2Index/genome [2019-04-30 23:03:29] Reading known junctions from GTF file [2019-04-30 23:03:34] Preparing reads left reads: min. length=76, max. length=76, 32092485 kept reads (7425 discarded) right reads: min. length=76, max. length=76, 32079587 kept reads (20323 discarded) [2019-04-30 23:23:19] Building transcriptome data files /scratch/5785970.1.linga/tophat2/tmp/RefSeq_GeneBody [2019-04-30 23:23:40] Building Bowtie index from RefSeq_GeneBody.fa [2019-04-30 23:32:14] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-05-01 00:17:19] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-05-01 01:05:17] Resuming TopHat pipeline with unmapped reads [2019-05-01 01:05:17] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2019-05-01 01:11:58] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/3) [2019-05-01 01:13:19] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/3) [2019-05-01 01:14:39] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/3) [2019-05-01 01:16:04] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2019-05-01 01:23:19] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/3) [2019-05-01 01:25:07] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/3) [2019-05-01 01:26:53] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/3) [2019-05-01 01:28:48] Searching for junctions via segment mapping [2019-05-01 01:48:37] Retrieving sequences for splices [2019-05-01 01:50:47] Indexing splices [2019-05-01 01:51:50] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/3) [2019-05-01 01:53:11] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/3) [2019-05-01 01:54:27] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/3) [2019-05-01 01:55:42] Joining segment hits [2019-05-01 01:58:40] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/3) [2019-05-01 02:00:14] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/3) [2019-05-01 02:01:34] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/3) [2019-05-01 02:03:07] Joining segment hits [2019-05-01 02:06:09] Reporting output tracks ----------------------------------------------- [2019-05-01 03:37:00] A summary of the alignment counts can be found in /scratch/5785970.1.linga/tophat2/align_summary.txt [2019-05-01 03:37:00] Run complete: 04:33:36 elapsed