-----------------------
Need shift 1 command (have more than 9 arguments):
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-----------------------
Start of variable list:
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Sample_ID:
KI5
Dataset_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/CAR_Project_Data/GSE96650_LxRKo
Sample_Labels_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/CAR_Project_Data/GSE96650_LxRKo/Scripts/00_Setup_Pipeline
GTF_Files_DIR:
/restricted/projectnb/waxmanlab/kkarri/mouse_gtf_files/final-RNAseq-Pipeline/GTF_Files
MODE:
intersection-nonempty
STRANDEDNESS_HTSeq:
reverse
FEATURE_ID:
gene_id
SCRIPT_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/CAR_Project_Data/GSE96650_LxRKo/Scripts/08a_Extract_Counts_HTSeq
ANNOTATION_FILE:
RefSeq_GeneBody.gtf
FEATURE_TYPE:
exon
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Tue Apr 30 05:10:38 EDT 2019
Running on node : scc-ka8
Current directory : /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/CAR_Project_Data/GSE96650_LxRKo/Scripts/08a_Extract_Counts_HTSeq
Current job ID : 5782407
Current job name : Step_08a_KI5
Task index number : undefined
Parameter for multiple cores : 1
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/5782407.1.linga

Loading required modules...


List files in scratch directory:

total 377M
drwx------   2 kkarri waxmanlab 4.0K Apr 30 05:23 .
drwxrwxrwt. 70 root   root       72K Apr 30 05:16 ..
-rw-r--r--   1 kkarri waxmanlab    0 Apr 30 05:23 KI5_HTSeq.out
-rw-r--r--   1 kkarri waxmanlab 306M Apr 30 05:18 KI5_primary_unique.bam
-rw-r--r--   1 kkarri waxmanlab  72M Apr 30 05:18 RefSeq_GeneBody.gtf

Starting to run my commands


Starting samtools and htseq-count

samtools view KI5'_sorted'.bam | htseq-count --stranded=reverse --type=exon --idattr=gene_id --order=name --mode=intersection-nonempty - RefSeq_GeneBody.gtf  > KI5_HTSeq.out

Ending samtools and htseq-count


List files in scratch

total 859M
drwx------   2 kkarri waxmanlab 4.0K Apr 30 05:29 .
drwxrwxrwt. 61 root   root       72K Apr 30 05:44 ..
-rw-r--r--   1 kkarri waxmanlab 236K Apr 30 05:47 KI5_HTSeq.out
-rw-r--r--   1 kkarri waxmanlab 306M Apr 30 05:18 KI5_primary_unique.bam
-rw-r--r--   1 kkarri waxmanlab 483M Apr 30 05:29 KI5_sorted.bam
-rw-r--r--   1 kkarri waxmanlab  72M Apr 30 05:18 RefSeq_GeneBody.gtf
==========================================================
Finished on : Tue Apr 30 05:47:49 EDT 2019
0 hours, 37 minutes and 11 seconds elapsed.
==========================================================