[2019-05-08 08:30:37] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2019-05-08 08:30:37] Checking for Bowtie Bowtie version: 2.2.2.0 [2019-05-08 08:30:37] Checking for Bowtie index files (genome).. [2019-05-08 08:30:37] Checking for reference FASTA file [2019-05-08 08:30:37] Generating SAM header for Bowtie2Index/genome [2019-05-08 08:30:40] Reading known junctions from GTF file [2019-05-08 08:30:42] Preparing reads left reads: min. length=101, max. length=101, 34562887 kept reads (242363 discarded) [2019-05-08 08:39:33] Building transcriptome data files /scratch/5992723.1.p16/tophat2/tmp/RefSeq_GeneBody [2019-05-08 08:39:42] Building Bowtie index from RefSeq_GeneBody.fa [2019-05-08 08:44:36] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-05-08 08:56:18] Resuming TopHat pipeline with unmapped reads [2019-05-08 08:56:18] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2019-05-08 09:02:45] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2019-05-08 09:03:28] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2019-05-08 09:04:15] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2019-05-08 09:05:03] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2019-05-08 09:05:57] Searching for junctions via segment mapping [2019-05-08 09:12:25] Retrieving sequences for splices [2019-05-08 09:13:32] Indexing splices [2019-05-08 09:14:05] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2019-05-08 09:14:22] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2019-05-08 09:14:38] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2019-05-08 09:14:54] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2019-05-08 09:15:11] Joining segment hits [2019-05-08 09:16:52] Reporting output tracks ----------------------------------------------- [2019-05-08 09:29:51] A summary of the alignment counts can be found in /scratch/5992723.1.p16/tophat2/align_summary.txt [2019-05-08 09:29:51] Run complete: 00:59:14 elapsed