[2019-05-08 09:18:05] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2019-05-08 09:18:05] Checking for Bowtie Bowtie version: 2.2.2.0 [2019-05-08 09:18:07] Checking for Bowtie index files (genome).. [2019-05-08 09:18:07] Checking for reference FASTA file [2019-05-08 09:18:07] Generating SAM header for Bowtie2Index/genome [2019-05-08 09:18:13] Reading known junctions from GTF file [2019-05-08 09:18:18] Preparing reads left reads: min. length=101, max. length=101, 39218651 kept reads (321276 discarded) [2019-05-08 09:39:47] Building transcriptome data files /scratch/5992731.1.linga/tophat2/tmp/RefSeq_GeneBody [2019-05-08 09:40:07] Building Bowtie index from RefSeq_GeneBody.fa [2019-05-08 09:48:26] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-05-08 10:18:12] Resuming TopHat pipeline with unmapped reads [2019-05-08 10:18:12] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2019-05-08 10:38:45] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2019-05-08 10:41:13] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2019-05-08 10:44:02] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2019-05-08 10:47:03] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2019-05-08 10:50:44] Searching for junctions via segment mapping [2019-05-08 11:05:43] Retrieving sequences for splices [2019-05-08 11:08:00] Indexing splices [2019-05-08 11:09:09] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2019-05-08 11:10:21] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2019-05-08 11:11:34] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2019-05-08 11:12:54] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2019-05-08 11:14:03] Joining segment hits [2019-05-08 11:17:48] Reporting output tracks ----------------------------------------------- [2019-05-08 11:42:46] A summary of the alignment counts can be found in /scratch/5992731.1.linga/tophat2/align_summary.txt [2019-05-08 11:42:46] Run complete: 02:24:40 elapsed