[2019-05-08 07:58:31] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2019-05-08 07:58:31] Checking for Bowtie Bowtie version: 2.2.2.0 [2019-05-08 07:58:32] Checking for Bowtie index files (genome).. [2019-05-08 07:58:32] Checking for reference FASTA file [2019-05-08 07:58:32] Generating SAM header for Bowtie2Index/genome [2019-05-08 07:58:36] Reading known junctions from GTF file [2019-05-08 07:58:40] Preparing reads left reads: min. length=101, max. length=101, 32465301 kept reads (249269 discarded) [2019-05-08 08:11:16] Building transcriptome data files /scratch/5992717.1.linga/tophat2/tmp/RefSeq_GeneBody [2019-05-08 08:11:40] Building Bowtie index from RefSeq_GeneBody.fa [2019-05-08 08:20:20] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-05-08 08:39:27] Resuming TopHat pipeline with unmapped reads [2019-05-08 08:39:27] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2019-05-08 08:53:32] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2019-05-08 08:54:47] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2019-05-08 08:56:23] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2019-05-08 08:57:58] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2019-05-08 08:59:32] Searching for junctions via segment mapping [2019-05-08 09:04:04] Retrieving sequences for splices [2019-05-08 09:06:11] Indexing splices [2019-05-08 09:06:50] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2019-05-08 09:07:11] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2019-05-08 09:07:37] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2019-05-08 09:08:00] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2019-05-08 09:08:23] Joining segment hits [2019-05-08 09:11:28] Reporting output tracks ----------------------------------------------- [2019-05-08 09:30:07] A summary of the alignment counts can be found in /scratch/5992717.1.linga/tophat2/align_summary.txt [2019-05-08 09:30:07] Run complete: 01:31:35 elapsed