[2019-05-08 08:18:15] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2019-05-08 08:18:15] Checking for Bowtie Bowtie version: 2.2.2.0 [2019-05-08 08:18:16] Checking for Bowtie index files (genome).. [2019-05-08 08:18:16] Checking for reference FASTA file [2019-05-08 08:18:16] Generating SAM header for Bowtie2Index/genome [2019-05-08 08:18:20] Reading known junctions from GTF file [2019-05-08 08:18:28] Preparing reads left reads: min. length=101, max. length=101, 34272289 kept reads (217664 discarded) [2019-05-08 08:38:49] Building transcriptome data files /scratch/5992720.1.linga/tophat2/tmp/RefSeq_GeneBody [2019-05-08 08:39:24] Building Bowtie index from RefSeq_GeneBody.fa [2019-05-08 08:52:20] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-05-08 09:24:43] Resuming TopHat pipeline with unmapped reads [2019-05-08 09:24:43] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2019-05-08 09:47:15] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2019-05-08 09:49:25] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2019-05-08 09:51:46] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2019-05-08 09:54:14] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2019-05-08 09:56:52] Searching for junctions via segment mapping [2019-05-08 10:01:33] Retrieving sequences for splices [2019-05-08 10:03:45] Indexing splices [2019-05-08 10:05:00] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2019-05-08 10:05:34] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2019-05-08 10:06:11] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2019-05-08 10:06:45] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2019-05-08 10:07:21] Joining segment hits [2019-05-08 10:12:46] Reporting output tracks ----------------------------------------------- [2019-05-08 10:47:16] A summary of the alignment counts can be found in /scratch/5992720.1.linga/tophat2/align_summary.txt [2019-05-08 10:47:16] Run complete: 02:29:00 elapsed