[2019-05-08 09:21:01] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2019-05-08 09:21:01] Checking for Bowtie Bowtie version: 2.2.2.0 [2019-05-08 09:21:02] Checking for Bowtie index files (genome).. [2019-05-08 09:21:02] Checking for reference FASTA file [2019-05-08 09:21:02] Generating SAM header for Bowtie2Index/genome [2019-05-08 09:21:08] Reading known junctions from GTF file [2019-05-08 09:21:13] Preparing reads left reads: min. length=101, max. length=101, 34874295 kept reads (214569 discarded) [2019-05-08 09:35:22] Building transcriptome data files /scratch/5992732.1.linga/tophat2/tmp/RefSeq_GeneBody [2019-05-08 09:35:44] Building Bowtie index from RefSeq_GeneBody.fa [2019-05-08 09:44:16] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-05-08 10:15:32] Resuming TopHat pipeline with unmapped reads [2019-05-08 10:15:32] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2019-05-08 10:36:17] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2019-05-08 10:39:23] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2019-05-08 10:42:47] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2019-05-08 10:46:09] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2019-05-08 10:50:01] Searching for junctions via segment mapping [2019-05-08 11:10:20] Retrieving sequences for splices [2019-05-08 11:12:30] Indexing splices [2019-05-08 11:13:58] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2019-05-08 11:15:18] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2019-05-08 11:16:40] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2019-05-08 11:17:59] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2019-05-08 11:19:17] Joining segment hits [2019-05-08 11:22:39] Reporting output tracks ----------------------------------------------- [2019-05-08 11:42:21] A summary of the alignment counts can be found in /scratch/5992732.1.linga/tophat2/align_summary.txt [2019-05-08 11:42:21] Run complete: 02:21:19 elapsed