[2019-04-29 17:43:04] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2019-04-29 17:43:04] Checking for Bowtie Bowtie version: 2.2.4.0 [2019-04-29 17:43:04] Checking for Bowtie index files (genome).. [2019-04-29 17:43:04] Checking for reference FASTA file [2019-04-29 17:43:04] Generating SAM header for Bowtie2Index/genome [2019-04-29 17:43:06] Reading known junctions from GTF file [2019-04-29 17:43:09] Preparing reads left reads: min. length=50, max. length=50, 84668143 kept reads (36519 discarded) right reads: min. length=50, max. length=50, 84578571 kept reads (126091 discarded) [2019-04-29 18:07:07] Building transcriptome data files /scratch/5765375.1.p16/tophat2/tmp/RefSeq_GeneBody [2019-04-29 18:07:16] Building Bowtie index from RefSeq_GeneBody.fa [2019-04-29 18:12:10] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-04-29 18:26:51] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-04-29 18:41:21] Resuming TopHat pipeline with unmapped reads [2019-04-29 18:41:22] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2019-04-29 19:02:19] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2019-04-29 19:05:25] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2019-04-29 19:08:38] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2019-04-29 19:30:42] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2019-04-29 19:35:04] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2019-04-29 19:41:28] Searching for junctions via segment mapping [2019-04-29 19:47:49] Retrieving sequences for splices [2019-04-29 19:48:49] Indexing splices [2019-04-29 19:49:03] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2019-04-29 19:50:14] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2019-04-29 19:51:20] Joining segment hits [2019-04-29 19:52:44] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2019-04-29 19:54:01] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2019-04-29 19:55:35] Joining segment hits [2019-04-29 19:57:29] Reporting output tracks ----------------------------------------------- [2019-04-29 20:28:34] A summary of the alignment counts can be found in /scratch/5765375.1.p16/tophat2/align_summary.txt [2019-04-29 20:28:34] Run complete: 02:45:29 elapsed