FastQCFastQC Report
Fri 5 Jun 2015
Waxman-TP19_GCCAAT_L007_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman-TP19_GCCAAT_L007_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCG250.005460586455.791845
CGGTTTA353.0943524E-453.1350864
ATTAGTA805.082877E-429.0582494
CGTGTGG700.00930198526.5675431
CCACGAT1050.002481900922.1396181
TACCATA650.00854077917.88557858-59
ACTTGCC1300.00853900417.8826
AAGATTA1300.00853900417.8823
CCAAGTC1650.001684562116.9066182
ATCAGCC2050.007143515613.6077656
CTCCCCT2100.00837750913.28377151
CTTCAAA2903.6638498E-412.825716
CAGGAAT2550.002036715912.7628391
CAAAGAT3751.828026E-512.3981871
AAACTCA2750.00358755411.8346324
CAACAAG2800.00410472911.6233011
AAGATGC2850.00468449611.4193823
CCCTCGG1550.00877495210.49846440-41
CAGCCAC3950.00483307349.4163451
GAAGAAG6253.424838E-48.1828031
Produced by FastQC (version 0.11.2)