FastQCFastQC Report
Fri 5 Jun 2015
Waxman-TP18_ACAGTG_L007_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman-TP18_ACAGTG_L007_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT2830.1132No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC503.2016647E-546.49223
CTTCGCG450.001068015441.32641
GCGACGT300.004377225431.00720222-23
CTCGTCT600.004381650630.99481
CACGATC753.483201E-430.99481
CGGAATA650.006479962728.6105842
CGAAGGA650.006479962728.6105848
CCGGAAT1100.00325144921.1328181
AATTGGA2802.0354491E-919.9252285
GATACAG1250.006811452618.596885
CTTGCCT1508.891717E-418.596881
ATTGGAC2252.034365E-618.596886
ATATAAT1300.00854006317.8816156
TCGGGGG952.1948057E-417.12870438-39
GAATTGG2505.5577475E-616.7371924
GGAATTG3351.8779247E-816.6539233
CAGATCT2356.137059E-515.8271313
CTGGAAT4802.910383E-1115.49741
GTAGAAA2104.693834E-415.4973998
ATCCAGT2104.693834E-415.4973996
Produced by FastQC (version 0.11.2)