FastQCFastQC Report
Fri 5 Jun 2015
Waxman-TP17_CGATGT_L007_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman-TP17_CGATGT_L007_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTT650.00648500228.6060312
CAGATGT1459.4734787E-725.6467883
AAAGGGG2155.619297E-415.1345878
AGATCGG3202.1645974E-1012.34999692-93
AGAATAT2800.00411027111.62123
GATTGGC3350.001232787911.1008475
GGGCTCT2950.006065035711.0302913
TATACTG1900.0035089289.7921550-51
Produced by FastQC (version 0.11.2)