FastQCFastQC Report
Wed 12 Oct 2016
DW-S2-3_S39_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S2-3_S39_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75432930
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACT1541043720.429323108621126Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTG67735528.97956900255631Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCAG28286973.7499497898331673Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTTTGAACTCCAG14361731.9039072193006423Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTTAACTCCAGTCAC13417111.778680743277505Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC11866441.5731113719167478Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGATCACACGTCTGAACT6595930.8744098896861093Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTTTTAACTCCAGTCAC4610920.6112608909663193Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGCAGTGGCTGGTTGAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGT4263320.5651802203626454Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCC4221530.5596401995786191Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTTTGAACTCCAGTCAC3969960.5262900433537449Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATCAGATCGGAAGAGCACACGTCTG3320720.4402215318959505Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAAGATCGGAAGAGCACACGTCTGAACTC3309220.4386969987775896Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACTTCTGAACTCCAG2768750.36704791925754443Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAAC2589030.34322278081999463Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAATAGCACACGTCTG2585190.34271371932655936Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTC2491960.33035439562005614Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACTC2161510.28654726788419854Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACT2080910.27586227924594736Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGATCACACGTCTG1982550.2628228812005579Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCCA1940590.2572603238399993Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA1899340.2517918898284874Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACTCCA1859910.24656472975396818Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC1837220.2435567596273935No Hit
AGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAA1789900.23728363726558147Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATAGATCGGAAGAGCACACGTCTGA1751360.23217446279761375Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGA1712310.22699767860004907Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACTCC1630390.21613770007342945Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACTTTTGAACTCCAG1620620.21484250976330893No Hit
AGAGCAGTGGCTGGTTGAGATTAAAGATCGGAAGAGCACACGTCTGAACTC1405630.1863416945357949Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTGGCTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT1305980.17313128364495453TruSeq Adapter, Index 8 (100% over 37bp)
AGCAGTGGCTGGTTGAGATTCAGATCGGAAGAGCACACGTCTGAACTCCAG1278310.16946312439408096Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGT1233390.163508165465666Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCCAG1161480.1539751935925066Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCC1148490.15225313400924503Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAACT963400.12771610488946936Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGCAGTGGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC862300.11431347025761826Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTGGCTGGTTGAGACAGATCGGAAGAGCACACGTCTTAACTCCAGTCAC858650.11382959670266024Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCCAGT855530.11341598423924405Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC840980.11148711842427438No Hit
AGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA802510.10638722372311403Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGCAATGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACT799350.1059683085358079Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTGGCTGGTTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA768390.10186400024498583Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
NGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACT766830.10165719401327776Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCAGTGGCTGGTTGAGATTCAGATCGGAAGAGCACACGTTTGAACTCCAG761890.10100230761286881Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGAGC9470250.044.783881
GAGAGCA9793700.043.27312
TCGTAAG963500.042.83835634
CGTTTTA617300.042.68525334
TAAGAGC948600.042.5343137
ACGTTTT619500.042.49242433
AGATCGT1015050.042.43681331
ATCGTAA971700.042.4292533
TTTTAAC611250.042.04528836
GTTTTAA614950.041.7989135
CGTAAGA977000.041.27747735
TTTAACT624800.041.1167337
GAGATAG4031200.040.9851513
ATTTAAA19471150.040.95131320
TTTAAAG19581150.040.74665521
CCCGCTA40150.040.6784926
CGCCCCG175050.040.5842366
TGAGATA4074750.040.5498712
GCGCCCC176100.040.4955525
TTAAAGA19760250.040.4225422
Produced by FastQC (version 0.11.2)