FastQCFastQC Report
Wed 12 Oct 2016
DW-S2-1_S37_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S2-1_S37_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85378412
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTG49914225.846234291638031Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACT40560014.750616584435888Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCAG17544342.05489181504102Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC10882721.2746453986518278Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTTAACTCCAGTCAC9520841.1151343503554505Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTTTGAACTCCAG7638830.8947027499176256Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA6239810.7308416558508959No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTCT5842390.6842935893443415Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCTG3845870.4504499334093963Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCTG3212860.37630824054211737Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTCT3178820.37232128421409383Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTTTGAACTCCAGTCAC3139430.3677077057839867Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTTTTAACTCCAGTCAC2792940.3271248474380151Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTCT2661690.31175210895231925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGTGACGTATGATGGATTCAATACATTAGATCGGAAGAGCACACGTCTG2519120.29505350837399036Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC2082240.2438836646434698No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATCAGATCGGAAGAGCACACGTCTG2050040.24011221946831246Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACAAGAAAGACATGGTCCTGACAGACAGAGATCGGAAGAGCACACGTCTG1894350.22187693066954678Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAATAGCACACGTCTG1832290.214608114285377Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGATCACACGTCTGAACT1736930.20343901453683633Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACTTCTGAACTCCAG1731290.20277842600305096Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC1689820.19792122626970388No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1683910.19722901381674796No Hit
AGCAGTGGCTGGTTGAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGT1585980.18575890120795407Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGA1539540.18031958711061527No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGATCACACGTCTG1461090.17113108170716504Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CATGTCTTGGAGAATTCCTTAGTTCTTAAAGATCGGAAGAGCACACGTCTG1456660.1706122151815145Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACAAGAAAGACGTGGTCCTGACAGACAGAGATCGGAAGAGCACACGTCTG1403620.164399871948895Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA1386390.16238179740330613Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAGATCGGAAGA1167980.13680038930684257No Hit
GAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAAC1140180.133544296888539Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1074590.12586202704262056No Hit
ACAAGAAAGACGTGGTCCTGACAGACAGAAGATCGGAAGAGCACACGTCTG1068170.12511008052012024Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTATACCTAAGACAGACTATGATAACTTAGATCGGAAGAGCACACGTCTG1058830.12401612716807149Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCC1057190.12382404114051689Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGGGGCTCTATGGCCGAGAACCAGATTTGAGATCGGAAGAGCACACGTCT1055550.1236319551129623Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT1041680.12200742267260722Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATAGATCGGAAGAGCACACGTCTGA1034290.12114186429234594Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGT967580.11332841374468292No Hit
AGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAA967300.11329561856924675Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT961860.11265845516077297Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGA954150.11175541658001323Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGACGTGGTCCTGACAGACAGACAATCAGATCGGAAGAGCACACGTCTG917770.1074943862858447Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC913840.10703408257347304No Hit
ACTGTAAGAGATGAAGAGTGCTCGGAATTAGATCGGAAGAGCACACGTCTG911920.10680920137048228Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAAGATCGGAAGAGCACACGTCTGAACTC900050.10541892018324256Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCTG896390.10499024039004146Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACTTTTGAACTCCAG889660.10420198492330825No Hit
ATTGATCATCGACACTTCGAACGCACTAGATCGGAAGAGCACACGTCTGAA870750.10198713932510248Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGAGC7154300.044.336581
GAGAGCA7367450.043.0463142
TCGTAAG1774800.042.62797534
TAAGAGC1752200.042.17833737
AGATCGT1881650.042.0465831
ATCGTAA1812500.041.7412633
CGTTTTA391950.041.5059934
CCCGCTA89900.041.21452726
ACGTTTT394500.041.17368733
ACGCACT576800.040.89990621
GTTTTAA387500.040.69420235
CGCACTT452800.040.69019722
CGAATCG57600.040.6578710
TTTTAAC389600.040.5098136
ACTTGCG371350.040.40720425
CTTGCGG372400.040.35368326
ATTTAAA5245950.040.34311720
CGTAAGA1834500.040.23680535
CGCCCCG198550.040.1777156
GAGATAG3203600.040.1425713
Produced by FastQC (version 0.11.2)