FastQCFastQC Report
Mon 13 Oct 2014
140923Wax_D14-6597_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename140923Wax_D14-6597_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences182673136
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC5616580.3074661180612786TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC3629040.19866303713097694TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1788150.040.10076543
CGTATGC1797400.039.84911344
CTCGTAT1816550.039.4006242
TCTCGTA1875050.038.1750741
ATCTCGT1932200.037.04709240
AATCTCG947600.032.71094539
CGTCTGA2269800.032.351216
ACGTCTG2337950.031.42135215
ACACGTC2426900.030.27267513
CACGTCT2468450.029.77891514
CAGTCAC2459200.029.727927
GAACTCC2492200.029.58476321
CACACGT2571550.028.63997712
TCTGAAC2610600.028.26662318
CCAGTCA2591650.028.23681826
CTGAACT2629950.028.10330419
TCCAGTC2612350.028.10237125
GTCTGAA2667300.027.68093717
TGAACTC2714350.027.25018720
AACTCCA2727550.027.22719422
Produced by FastQC (version 0.11.2)