FastQCFastQC Report
Wed 19 Oct 2016
DW-S1-2_S33_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S1-2_S33_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84154773
Sequences flagged as poor quality0
Sequence length20-51
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGCAGTGGCTGGTTGAGATTTAA21406942.543758272629409No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATT18506882.1991479912850576No Hit
AGCAGTGGCTGGTTGAGATTT11916051.4159684085892548No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT8050390.9566171606214183No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC7268580.8637157158037846No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT6433910.7645329873327565No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC4061640.48263929129723876No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACG3083830.36644742657674334No Hit
TTTGGTGACTCTAGATAACCTCGGG3023490.3592773044495052No Hit
ATTGATCATCGACACTTCGAACGCA2970950.3530340459714626No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC2880580.3422954987948218No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2774660.3297091657534386No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT2458640.29215692852026354No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG2225610.2644662828571827No Hit
TCTGTGACGTATGATGGATTCAATACATTT2133840.25356137553837854No Hit
ATCACAAGAAAGACGTGGTCCTGACAGAC1990920.2365783815969654No Hit
TATTCTGTGACGTATGATGGATTCAATAC1959120.2327996297963991No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT1954550.23225658276090888No Hit
CCGTGGCGCAATGAAGGTGAAGGGC1890250.22461589908869456No Hit
ATTGATCATCGACACTTCGAACGCACT1801990.21412808041202844No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACA1624190.19300034235728972No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT1616400.19207466699482392No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1577000.18739281727965684No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG1575130.1871706076611959No Hit
CACAAGAAAGACATGGTCCTGACAGACAG1540160.18301516896730266No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA1539040.18288208085357202No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTG1523770.18106756701726234No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG1464050.17397111866726797No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCG1335660.1587147053441639No Hit
TTTGGTGACTCTAGATAACCTCGGGC1316430.15642962996287804No Hit
TCTGTGACGTATGATGGATTCAATACATT1255290.1491644448972609No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCG1161430.13801118565194156No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA1146070.13618597723506426No Hit
TTTGGTGACTCTAGATAACCTCGGGCCG1128620.13411241689167175No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTT1120490.13314633978039486No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCT1100200.13073530600575678No Hit
TTTGGTGACTCTAGATAACCTCGGGCC1070390.12719302326440832No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGA1069560.12709439546584006No Hit
AGCAGTGGCTGGTTGAGATT1048780.12462513564144484No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1031540.1225765293193768No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1005210.11944777035997708No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATC985160.11706525546685272No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT969650.11522222274902934No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGC966520.1148502890026214No Hit
CATTGATCATCGACACTTCGAACGCA966130.11480394582016162No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT896930.10658100165037579No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC858380.10200015630723644No Hit
CATGTCTTGGAGAATTCCTTAGTTCTTAA856330.10175655752764018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAATAG136800.0695.4963437
CATACGT73350.0665.3390543
ATACGTC110900.0662.148344
ACGTCTG811800.0642.911545
CGTAAGT46350.0616.232435
GTAAGTG43250.0616.2236336
GAATAGC166750.0587.8575438
CGTAATA95050.0563.7449335
TACGTCT144550.0562.399345
TAGAGTA91600.0560.051138
CACTCGT41600.0547.622342
CCCGGTT25950.0531.756434
GCATACG60200.0527.499242
ACTCGTC59800.0521.395543
GTAATAT24050.0508.5359536
GTATACG24650.0497.5097442
CGGCCCC292950.0496.7342830
TAGATCT190700.0491.443931
CGTAAGA106650.0490.992535
TCGTAAG155300.0487.06234
Produced by FastQC (version 0.11.2)