FastQCFastQC Report
Mon 8 Sep 2014
140902Wax_D14-5987_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename140902Wax_D14-5987_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences145169342
Sequences flagged as poor quality0
Sequence length45
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG12312350.8481370673981563TruSeq Adapter, Index 7 (100% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG11885510.8187341649588795TruSeq Adapter, Index 9 (100% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG10253040.7062813579467764TruSeq Adapter, Index 8 (100% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA4879950.038.478421
ATCGGAA4899750.038.3164832
CGTCTGA4891250.038.0646216
ACACGTC4901900.038.01351513
ACGTCTG4907850.037.92280215
TCGGAAG4960750.037.847553
CACGTCT4941300.037.68714514
CGGAAGA4982500.037.6792074
CACACGT4995350.037.2871712
GCACACG5013100.037.28612511
CGATCAG1294600.036.9769433
ACGATCA1299100.036.9666932
GTCACGA1332350.036.89217429
AATCTCG1205250.036.81210739
TCACGAT1319150.036.7210630
GATCTCG1344000.036.70350339
CACGATC1312650.036.66264731
TATCTCG878900.036.2184939
GAACTCC5179850.036.0589321
CATCTCG1434350.035.88789439
Produced by FastQC (version 0.11.2)