FastQCFastQC Report
Wed 23 Nov 2016
G141-M9-Index-22_CGTACG_BC9WPLANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG141-M9-Index-22_CGTACG_BC9WPLANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23701055
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC618480.2609504091695496No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG597140.25194659056316265No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT373120.1574275913034251No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG372680.1572419455589635No Hit
CGTCGTCCCCCGCGTCGTCGCCACCTCTCTTCCCCCCTCCTTCTTCCCGT304120.12831496319467636No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT291360.12293123660529034No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC290470.12255572589490214No Hit
GCCCTCCCCTCTTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCAC256710.10831163422894044No Hit
CGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCC239920.10122756138914492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC134850.025.9520991
CACGATC49300.025.5498351
TGGCGCA143300.022.7971484
ACGATCA56550.022.351962
GGTGGCG172600.020.1868652
GTGGCGC171100.020.0935233
CGATCAC63150.019.9805723
GCGCACG204700.015.9713436
CGCGGTG31550.013.8654541
GGCGCAC250500.013.4451415
CGCACGC244850.013.3330277
GCCCTCT259450.012.971523
GCACGCC261350.012.520418
CGGCGTA15900.011.890754
TCACAAG115100.011.7857756
CCACGAT24150.011.6512681
CACGCCT289050.011.4344149
GATCACA117350.011.3713894
CGCCTGT306550.011.23297411
ACGCCTG296050.011.20117810
Produced by FastQC (version 0.11.2)