FastQCFastQC Report
Wed 23 Nov 2016
G141-M9-Index-22_CGTACG_BC9WPLANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG141-M9-Index-22_CGTACG_BC9WPLANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23701055
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG853510.3601147712622919No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA802360.3385334534686325No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC386180.16293789453676216No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA288600.1217667314809404No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT277230.11696947667519443No Hit
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA266170.11230301773486455No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA249370.10521472567360397No Hit
GCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAAT249020.10506705292232772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT47100.022.7323651
CTCGCTA104350.020.3350351
TCGCTAT115400.018.5930482
CCTCCCG293300.018.5571244
CGCTATG126050.016.8336723
CGCTGGT341450.015.84998536
CCCGAGT321100.015.5495981
GGTTCCT356300.015.37609440
TCCTCCC361100.015.30015143
TGGTTCC360300.015.22328739
GTTCCTC361900.015.18567741
GCCCGCT363600.014.93458433
GCGGGTA29450.014.8797131
GCTGGTT368950.014.76195737
CCGCTGG369650.014.65946635
CCCGCTG369250.014.65363834
GGCCCGC374350.014.52877732
CCGAGTG340550.014.4772232
TGTCCGG380200.014.4757687
GTGTCCG383250.014.4120616
Produced by FastQC (version 0.11.2)