FastQCFastQC Report
Wed 23 Nov 2016
G141-M12-Index-27_ATTCCT_BC9WPLANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG141-M12-Index-27_ATTCCT_BC9WPLANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8514244
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC249500.29303834844291515No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG151380.17779617309534468No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT129840.1524973914301728No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG122180.14350070305713578No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC120110.14106948309209838No Hit
GGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCC113900.13377582319698614No Hit
GCCCTCCCCTCTTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCAC113130.13287145635008815No Hit
CGTCGTCCCCCGCGTCGTCGCCACCTCTCTTCCCCCCTCCTTCTTCCCGT107310.12603585239041776No Hit
CGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCC106670.12528417085533372No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT93430.10973375909828283No Hit
CGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCCA91550.1075256945889735No Hit
GCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCC90340.10610454668670524No Hit
CGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGC86340.10140653709243005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC42850.020.4188821
TGGCGCA40500.019.7593024
CGCGGTG12600.015.8770781
GTGGCGC52600.015.672293
GGTGGCG54550.015.5960972
CGGCGTA4950.014.6566264
GGCGTAA5350.013.9720675
GCGTAAA6100.012.9760936
GCGCACG63450.012.5790046
GCCCTCT99150.012.5712153
CCGGCGG27850.011.8403841
GGCGCAC72650.011.6206685
GGTGCCG76100.011.1503783
TCGGCGT6750.011.0729193
TCCCACG115150.010.9357189
CGTAAAA7050.010.9138677
TGCCGGC78600.010.8528715
GTGTCCG63950.010.53533129
CCCACGT119550.010.50761710
TCCGCAC63250.010.50426732
Produced by FastQC (version 0.11.2)