FastQCFastQC Report
Wed 23 Nov 2016
G141-M11-Index-25_ACTGAT_BC9WPLANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG141-M11-Index-25_ACTGAT_BC9WPLANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9993865
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC303500.3036863115521372No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG176940.1770486193279577No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC142960.1430477598006377No Hit
CGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCCC141890.14197710295266147No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT140450.1405362189703383No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG131550.1316307554684799No Hit
GCCCTCCCCTCTTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCAC124910.12498667932776758No Hit
CGTCGTCCCCCGCGTCGTCGCCACCTCTCTTCCCCCCTCCTTCTTCCCGT122830.12290540246441192No Hit
GGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCC113420.11348962588548074No Hit
CGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCC112400.11246899973133519No Hit
CGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGC103050.10311325998500079No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT100490.10055168846087074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC45150.023.2246931
TGGCGCA47750.020.3498954
CGTCGGA7450.019.51097926
ATCGTCG6700.018.40791524
GGTGGCG59950.017.1611372
GTGGCGC59650.017.0991743
CGGCGTA9350.014.3427214
GATCGTC8400.013.89405623
GCGCACG71050.013.6159416
GGCGTAA10250.013.5125945
GCCCTCT120050.013.476693
CGCGGTG13650.013.0449291
GCGTAAA10150.012.9970346
CGTAAAA12000.011.9080017
TCGGCGT11650.011.6985863
ATGATCG7400.011.60245221
TGATCGT7500.011.4496522
TCGGACT10800.011.41966728
TTCGGCG12150.011.3986562
CGCACGC84900.011.3674647
Produced by FastQC (version 0.11.2)