FastQCFastQC Report
Wed 23 Nov 2016
G141-M10-Index-23_GAGTGG_BC9WPLANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG141-M10-Index-23_GAGTGG_BC9WPLANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24555883
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC694270.28273061897224383No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG633920.2581540236203275No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG486570.1981480364603464No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT344520.14030039155993698No Hit
CGTCGTCCCCCGCGTCGTCGCCACCTCTCTTCCCCCCTCCTTCTTCCCGT337910.13760857225130124No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC330540.13460725480733068No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT329030.13399233088054704No Hit
GGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCC316980.12908515649793575No Hit
GCCCTCCCCTCTTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCAC291490.11870475193256134No Hit
CGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCC273000.11117498808737604No Hit
GCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGC262770.10700898029201394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC142100.024.565791
CACGATC29650.021.4263171
TGGCGCA155050.020.898934
GGTGGCG188000.018.2991472
GTGGCGC191100.017.7947733
CGATCAC35350.016.9760273
ACGATCA37200.016.1322172
CGCGGTG36050.014.0248081
GCGCACG238150.013.5986186
CGGCGTA23900.013.5212774
GCCCTCT300550.013.0332833
GGCGTAA25350.013.0082715
GCGTAAA26000.012.5150036
TTCGGCG28500.011.7239272
GGCGCAC299350.011.4418045
TCGGCGT28850.011.3528083
CGCACGC287800.011.2592037
GGCCTTA73150.011.209674
CGTAAAA30550.010.9378847
TCCCACG354500.010.8921569
Produced by FastQC (version 0.11.2)