FastQCFastQC Report
Wed 23 Nov 2016
G140-M8-Index-9_GATCAG_BC9WPLANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140-M8-Index-9_GATCAG_BC9WPLANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24262966
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT14200.016.3198621
GGGCGAT10950.013.5272851
GGCGATC10600.013.0659162
ATCACGA32050.011.71891114
CGCGTCA19200.011.4419813
TCACGAA32550.011.4109715
GCGATCT12850.011.1496893
GATCACG34750.010.98595413
GCGACAT24450.010.8721997
AGGGCGA41000.010.2441434
CGAACGG3059.277901E-79.59377137
ACGAAGG39200.09.58898417
TGCGACA26250.09.5747026
GACGATC9150.09.3950492
CGAAGGT40100.09.37363318
CACGAAG39900.09.36534316
GGGCGAG43550.09.35721435
GGGAGAT75550.09.328691
GCGAGGC44000.09.16781337
CGATCTG17250.09.1340394
Produced by FastQC (version 0.11.2)