FastQCFastQC Report
Wed 23 Nov 2016
G140-M8-Index-9_GATCAG_BC9WPLANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140-M8-Index-9_GATCAG_BC9WPLANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24262966
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGT57300.019.40168625
TACGTTC56900.019.35402127
CTACGTT59850.018.5392626
CCCTACG65500.017.03330424
CCGCCGA12150.016.834724
ACGTTCT67000.016.76915228
GACTACC69200.015.50239519
CCCGCCG13250.015.43710123
GTCGGAT7650.015.0332161
ACTACCC73200.014.78674120
CTCGGGT20400.014.6808761
CTCGGTT20650.014.6191671
ACCCTAC78400.014.41510223
CTCGGAT17650.014.1175951
CTCGCGT2751.6189006E-1013.9398921
CCCGGTT11700.013.7202191
CGGGCAT23500.013.6618351
TACCCTA79300.013.59747322
ATGGACC83550.013.5923641
CGCCCGT2651.3897079E-913.5618051
Produced by FastQC (version 0.11.2)