FastQCFastQC Report
Wed 23 Nov 2016
G140-M10-Index-11_GGCTAC_BC9WPLANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140-M10-Index-11_GGCTAC_BC9WPLANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22938767
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCGA12350.021.24059724
CCTACGT47450.019.11343825
TACGTTC48900.018.754627
CCCGCCG14350.018.42409323
CTACGTT50500.018.08048626
CGCCCGC16150.016.40286821
GACTACC56000.016.29629919
ACGTTCT57900.016.11967328
CCCTACG57700.015.96573724
GTCCGCG31700.014.7817144
ACTACCC63100.014.46275320
CTCGGTT17600.014.4444971
CCCGTAT6050.014.2710641
CTCGCGT3053.6379788E-1214.1540881
ACCCTAC66650.013.94570323
GCCCGCC19550.013.86806622
CCCGGTT10900.013.8619171
CGTCCGC22600.013.70088143
CGCGTCG7450.013.62359340
CTCGGGT22250.013.4737731
Produced by FastQC (version 0.11.2)