FastQCFastQC Report
Wed 23 Nov 2016
G139-M7-Index-1_ATCACG_BC9WPLANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG139-M7-Index-1_ATCACG_BC9WPLANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17588808
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG603860.3433205934137208No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA572440.32545696104022515No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC306330.1741618874911819No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG304090.17288835036461822No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA211080.12000813244422248No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA198630.11292976761131283No Hit
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC193250.10987100433411975No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT192380.10937637161085617No Hit
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA186140.10582866104400024No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT179080.10181474492188443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT33950.025.3854981
CCCCCGT14100.023.09610616
ATCGGCG5550.019.48014522
CGACCCA17000.019.45681420
TCGCGAT4700.018.4339542
CCTCCCG295750.018.26812744
CTCGCTA118550.017.9967651
CCCGATT9300.016.620291
CCGATTG7800.016.2111512
TCGCTAT136050.016.0238112
CGTCCCA17000.015.74368120
TCCTCCC346050.015.71042443
TACGTTC24750.015.45591227
TGATCAA57450.015.4035434
GGTTCCT351200.015.30603340
ATCGACG2653.8198777E-1115.24672721
GTTCCTC355500.015.22185741
TGGTTCC354650.015.11544739
CGCTGGT358000.014.93635836
GCTGGTT361500.014.861876537
Produced by FastQC (version 0.11.2)