FastQCFastQC Report
Wed 23 Nov 2016
G139-M13-Index-8_ACTTGA_BC9WPLANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG139-M13-Index-8_ACTTGA_BC9WPLANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19154234
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG777810.4060773195106627No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA754490.3939024656376235No Hit
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA564070.2944884144153193No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA518850.27088005711948593No Hit
CCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCAC453720.2368771311867653No Hit
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC432910.22601269254620154No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG425680.22223807018333389No Hit
GGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACACG370790.19358122073688774No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC353200.18439787255392204No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA332680.17368483647009847No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT302260.15780323034583374No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT236040.12323123963088266No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT223430.11664783880159342No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA217170.11337963188713263No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT206320.10771508795392185No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC203210.1060914260523287No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG198820.10379950458995124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGT15850.029.62171716
CGTTGAT60100.025.7048441
CGACCCA18900.024.84606620
CCGGCGT12600.022.23714316
TCGCGAT4850.021.2327462
CGGCGTG14100.020.94725617
CGTCCCA20100.020.72499320
TTACTCG7300.019.71326320
CCCCGTC22600.019.43574717
GCCCGGG239200.019.3753471
TGATCAA84250.019.1636244
CCTCCCG392250.017.98552544
CCGTTGA24800.017.6496221
TTGATCA91700.017.5557793
CCGATTG8350.017.3780272
GATCAAA95750.017.3522825
ATCGACG3350.017.33606521
CTCGCGA6000.017.1651231
CGGCGGT10750.017.14118816
ATATATC99000.016.98933612
Produced by FastQC (version 0.11.2)