FastQCFastQC Report
Wed 23 Nov 2016
G139-M11-Index-6_GCCAAT_BC9WPLANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG139-M11-Index-6_GCCAAT_BC9WPLANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21212063
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG875340.4126614181751204No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA870000.41014398269512964No Hit
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA437590.2062929946983469No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC424410.20007954907544823No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG424270.20001354889432488No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA399310.18824665945976118No Hit
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC352870.16635345652141426No Hit
CCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCAC346640.163416448461425No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT267620.12616406051594323No Hit
GGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACACG266830.12579163092246143No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT249280.11751803678878381No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA228550.10774529568387574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGT17150.029.42448816
TCGCGAT5350.025.7959482
CGTTGAT50100.024.0013961
ATCGGCG5300.023.24085622
CGACCCA20300.022.74967220
CGTCCCA19850.022.50260720
ATCGACG3350.020.3420821
CCCGATT10300.019.0788921
CCCCGTC24750.019.0652517
CCTCCCG418300.018.8906844
GCCCGGG190850.018.8772931
CCGGCGT11450.016.96790916
TGATCAA71100.016.775294
CTCGCTA181100.016.6899551
CCGATTG10300.016.5782592
CCGGGCT58700.016.4995981
CTCGCGA7100.016.4748971
TCCTCCC486400.016.3935243
CCCGTCC29200.016.33304818
TCGAGTG16400.016.2597662
Produced by FastQC (version 0.11.2)