FastQCFastQC Report
Wed 23 Nov 2016
G126-G127-M1M20-NEBNext27_ATTCCT-_AC8KUAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext27_ATTCCT-_AC8KUAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7830323
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2092142.671843805166147No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1240891.5847238996398998No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA749850.9576233317578342Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT700130.8941265896694173No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGA653020.8339630434146842Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT562970.7189614017199546No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG544550.6954374679052192No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC535980.6844928363746936No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC502290.6414677913031174No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG475830.6076760818168038No Hit
CTCGCCCGTCACGTTGAACGCACGTTAGATCGGAAGAGCACACGTCTGAA413470.5280369660357561Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG389600.49755291065260016No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG381820.48761717747786393Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC367380.46917604803786517Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT299730.3827811445326074No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC287940.36772429438734516No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC287660.3673667101599768No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT273980.3498961664799779No Hit
CTAGACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA235160.3003196675284021Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC219080.27978411618524546Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA186310.2379339907178797No Hit
CTAAACCATTCGTAGACGACCTGCTAGATCGGAAGAGCACACGTCTGAAC183610.23448585709682732Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGCCCGTCACGTTGAACGCACGTAGATCGGAAGAGCACACGTCTGAAC181160.23135699510735383Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCCGTCGCCGCGCTCTCCCTCTCCCCAGATCGGAAGAGCACACGTCTG177590.22679779620840673Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA174600.22297930749472275No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCAGATCGGAAGAGCACACGTCTG173160.22114030289682815Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA158620.20257146480419774Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA154870.1977823903305138No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG140120.17894536406735712Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAGACCATTCGTAGACGACCTGCTAGATCGGAAGAGCACACGTCTGAAC127610.1629690116231476Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT127390.16268805258735813No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT124940.15955919059788465No Hit
CTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG114620.14637965764630656No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG110940.14167997922946474No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT106750.1363289866842019No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTAGATCGGAAGAGCACACGTCT101140.1291645312715708Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGAGAAGACGGTCGAACTTGACTATCAGATCGGAAGAGCACACGTCTG100540.12839827935578135Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA99000.12643156610525516No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT82410.10524470063367757Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC82320.10512976284630915No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGC81700.1043379691999934No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAACC189550.043.624161
GTTGGGG202600.042.7227551
ATTCGTA300300.042.518478
AACCATT216950.042.5074124
ACCATTC302100.042.4747965
TAAACCA199250.042.294892
CTCGCCC290750.042.2665331
AAACCAT205950.042.1916853
CGTGGGG431300.042.0695571
CTTGGGG133900.042.0549661
CGTAGAC301750.042.04941611
ACTTGCG149100.042.0168725
TCGCCCG289100.041.9534572
GACGACC294300.041.91650415
TAGACGA305550.041.88954513
GCCGTGA63600.041.754381
ACGACCT294400.041.74559416
AGACGAC299500.041.710314
CTAGACC79000.041.6037331
TTGCGGC148300.041.6014327
Produced by FastQC (version 0.11.2)