FastQCFastQC Report
Wed 23 Nov 2016
G126-G127-M1M20-NEBNext25_ACTGAT-_AC8KUAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext25_ACTGAT-_AC8KUAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7059706
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA6896799.769231183281569No Hit
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGATCGTCGGACTGTAGAAC1370931.9419080624603915No Hit
GCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAAC985161.3954688764659604No Hit
GAACGTGCGTTCAACGTGACGGGCGAGGATCGTCGGACTGTAGAACTCTG612810.8680389806601012Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCTCGATCAGAAGGACTTGGGCCCCCCACGATCGTCGGACTGTAGAACT442220.6264000228904717No Hit
GGCCTCGATCAGAAGGACTTGGGCCCCCCCACGATCGTCGGACTGTAGAA405110.5738340945076182No Hit
GCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGAACT307990.43626462631729984No Hit
GCCCTTCACCTTCATTGCGCCACGGGATCGTCGGACTGTAGAACTCTGAA257530.3647885620166052Illumina DpnII expression Sequencing Primer (100% over 25bp)
AAGCAGGTCGTCTACGAATGGTTTAGGATCGTCGGACTGTAGAACTCTGA243890.34546764412002423Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGCCTCGATCAGAAGGACTTGGGCCCCCACGGATCGTCGGACTGTAGAAC233210.3303395353857512No Hit
GCCTCGATCAGAAGGACTTGGGCCCCCCCACGATCGTCGGACTGTAGAAC195800.27734866012833964No Hit
GGGGAGAGAGAGAGCGCGGCGACGGGTAGATCGTCGGACTGTAGAACTCT195410.27679622919141394Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC190960.27049285055213346No Hit
CGAACGTGCGTTCAACGTGACGGGCGAGGATCGTCGGACTGTAGAACTCT189100.26785817992987243Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGTACTGTAGAA173660.24598758078594207No Hit
AACGTGCGTTCAACGTGACGGGCGAGGATCGTCGGACTGTAGAACTCTGA159950.22656750861863084Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGGGAGAGGGAGAGCGCGGCGACGGGTAGATCGTCGGACTGTAGAACTCT149810.2122043042585626Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA149460.21170853290491135No Hit
GCAGAGTACTAACCACTATACGATCACGGATCGTCGGACTGTAGAACTCT120120.17014872857311622Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGATCGTCGGACTGTA96540.13674790423283917No Hit
GAAGCAGGTCGTCTACGAATGGTTTAGGATCGTCGGACTGTAGAACTCTG96530.13673373933702054Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCTCGATCAGAAGGACTTGGGCCCCCCCGATCGTCGGACTGTAGAACT94840.13433987194367583No Hit
GGGAGAGAGAGAGCGCGGCGACGGGTAGATCGTCGGACTGTAGAACTCTG90890.12874473809532577Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGCCTACAGCACCCGGTATTCCCAGGCGGTGATCGTCGGACTGTAGAAC90250.1278381847629349No Hit
GGCCTCGATCCGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA89460.12671915799326489No Hit
GATAGTCAAGTTCGACCGTCTTCTCAGCGATCGTCGGACTGTAGAACTCT78040.11054284696841482Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGGGAGAGAGAGAGCGCGGCGACGGGTGATCGTCGGACTGTAGAACTCTG74760.1058967611399115Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAACT73110.10355955332984122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTCG1770950.043.5724451
TGCCTCG41800.040.525481
CTCGATA48550.039.3269844
CGATAAG45250.039.02336
TCGATAA47200.039.0043225
AGAGTAC99750.038.8473853
CTTAGTA12900.038.714848
AAGCCTA18900.038.5784071
GGCCTCT47250.038.5084761
GAGTACT99550.038.292754
GTGCGAT66500.037.9281731
GATAAGA44600.037.8641937
GGCATCG40750.037.6235471
AGCGACG54700.037.57271
ATGAGAG29900.037.352081
GCGACGC57900.037.289712
GTACTAA105150.037.146046
TACTAAC104950.037.1119357
GCAGAGT105200.037.0416341
ACTAACC102600.036.9041188
Produced by FastQC (version 0.11.2)