FastQCFastQC Report
Wed 23 Nov 2016
G126-G127-M1M20-NEBNext22_CGTACG-_AC8KUAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext22_CGTACG-_AC8KUAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8934830
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA118135413.221896779233628No Hit
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGATCGTCGGACTGTAGAAC2538812.841475439376015No Hit
GCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAAC1451931.6250225242114287No Hit
GCCTCGATCAGAAGGACTTGGGCCCCCCACGATCGTCGGACTGTAGAACT753870.8437429699277995No Hit
GCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGAACT339570.3800519987509555No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGATCGTCGGACTGTA311910.3490944987201771No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAGATCGTCGGACTGTAG301750.33772326949701337No Hit
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGTACTGTAGAA297210.33264203124178077No Hit
TGCGATCGGCCCGAGGTTATCTAGAGTCACCAAGATCGTCGGACTGTAGA263200.294577512946525No Hit
TGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA260930.29203689381890874No Hit
AAGCCTACAGCACCCGGTATTCCCAGGCGGTGATCGTCGGACTGTAGAAC239040.26753726707726955No Hit
GGCCTCGATCAGAAGGACTTGGGCCCCCACGGATCGTCGGACTGTAGAAC190090.21275166958968444No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC177390.19853763306072977No Hit
AATCCCCGATCCCCATCACGAATGGGGTTCAACGATCGTCGGACTGTAGA162270.18161509508295065No Hit
GGCCTCGATCCGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA161250.1804734952987354No Hit
GGGGAGAGAGAGAGCGCGGCGACGGGTAGATCGTCGGACTGTAGAACTCT155360.17388131615262967Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCTCGATCAGAAGGACTTGGGCCCCCCCACGATCGTCGGACTGTAGAA126580.14167029479016388No Hit
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTATAA117180.131149669327788No Hit
GGCCTCGATAAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA115690.12948203827045393No Hit
GGGGAGAGGGAGAGCGCGGCGACGGGTAGATCGTCGGACTGTAGAACTCT115050.1287657403666326Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA107540.1203604321514791No Hit
AGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA106060.11870399324889226No Hit
CCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAACT102050.11421593919526168No Hit
CGAACGTGCGTTCAACGTGACGGGCGAGGATCGTCGGACTGTAGAACTCT100940.11297361001832155Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCCCGCCTCACCGGGTCAGTGAAAAAACGGATCGTCGGACTGTAGAACTC95260.1066164661219072Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACGGTAGAA93600.10475856843387059No Hit
GGCCTCGATCAGAAGGACTTGGGCCCCCCAGATCGTCGGACTGTAGAACT93090.10418776854176297No Hit
AATGTATTGAATCCATCATACGTCACAGAGATCGTCGGACTGTAGAACTC92540.1035722000306665Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCTTCGGACTGTAGAA91990.10295663151957005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTCG2925550.043.65551
GTGCGAT176750.042.3457871
TGCCTCG73850.041.267111
AAGCCTA49200.041.1309661
CGATAAG70850.039.960366
TCGATAA75350.039.8035745
CTCGATA77100.039.7295074
GGCATCG64550.039.6395151
GGCCTCT74350.039.2125851
TTAGTAG16700.038.469539
GTCCTCG66250.038.024071
GGCCTAG21850.037.993421
AGAGTAC90600.037.8639643
ATTTGGG40000.037.57056417
CTTGCGC48550.037.56611318
GATAAGA71550.037.5392657
ACTTGCG45600.037.4441417
TTGAGCC56350.037.4410819
TTTGGGC47250.037.34251418
GACTTGC46650.037.26607516
Produced by FastQC (version 0.11.2)