FastQCFastQC Report
Wed 23 Nov 2016
G126-G127-M1M20-NEBNext22_CGTACG-_AC8KUAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext22_CGTACG-_AC8KUAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8934830
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG95908610.734238927881112No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2465862.75982867049513No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2201202.4636171029555123No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1741291.9488787139766508No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1269241.4205530491346785No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT985731.1032442698965732No Hit
CGTGTGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG814550.91165696493386No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC551900.6176950204984314No Hit
CTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG517420.5791044709300568No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC414200.4635790496293718No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC313300.35065020823003906No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT285290.3193009827831083No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT284330.3182265359273764No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG262850.2941857875303727No Hit
CGGGTGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG251000.2809230841549307No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC246930.27636787717281697No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT220040.24627217305757354No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA212700.2380571314731226No Hit
CGCGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG210400.23548293588126468No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC188280.21072588958043972No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCAGATCGGAAGAGCACACGTCTG187700.2100767446051016Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT181730.20339502822101818No Hit
CGTCGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG172130.19265055966369815No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC162260.18160390292820344No Hit
CGAGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG154400.17280686929689765No Hit
CGTGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT150950.16894557590911075No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG148680.16640495678149445Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACCCGTCGCCGCGCTCTCCCTCTCCCCAGATCGGAAGAGCACACGTCTG135210.15132912433700474Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGA128850.14421091391778018Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG123320.13802165234257394No Hit
TGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG123300.13799926803307955No Hit
NTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG116770.13069079098315245No Hit
CATGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG114150.12775844643938386No Hit
NGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG110490.12366211780190557No Hit
TTTGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA108500.12143487900721112No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA104220.11664463677540592Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGTGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT103390.11571568793138762No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT101830.1139697117908231Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT100370.11233565719773067No Hit
TTTGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC100040.1119663160910728No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACG96970.10853032458367984No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGAGATCGGAAGAGCACACGTC92240.10323643538825025No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG90690.10150165140243295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGGG468100.043.624161
CGTGGGG1602650.043.2579731
CGTGTGG150750.042.9224241
GTTGGGG242500.042.51641
GCCGTGA74550.042.0859641
TTGGGGG745550.041.846312
ACCGCCT61600.041.7352371
GTGTGGG157450.041.503182
TTGTGGG93100.041.3159262
CTAAACC36550.041.2185331
CTTGTGG74650.040.928241
TCGCCCG60100.040.8514822
CGTCACG68200.040.839897
ACCGGGT70950.040.65233214
TACCGGG71200.040.5377713
CGGGTGG58800.040.510511
GTCACGT68150.040.3587078
AACCATT41650.040.2700734
AATACCG72450.040.2690211
GTAGGCT71300.040.24960324
Produced by FastQC (version 0.11.2)