FastQCFastQC Report
Wed 23 Nov 2016
G126-G127-M1M20-NEBNext21_GTTTCG-_AC8KUAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext21_GTTTCG-_AC8KUAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8449621
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA101257511.983673587253204No Hit
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGATCGTCGGACTGTAGAAC2564803.0354024162740556No Hit
GCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAAC1687151.996716775817519No Hit
GCCTCGATCAGAAGGACTTGGGCCCCCCACGATCGTCGGACTGTAGAACT986891.1679695456163064No Hit
GCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGAACT355490.42071709488508424No Hit
GGGGAGAGAGAGAGCGCGGCGACGGGTAGATCGTCGGACTGTAGAACTCT264650.31320931435859667Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGTACTGTAGAA252030.29827373322424755No Hit
TGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA227720.26950321203755767No Hit
AAGCCTACAGCACCCGGTATTCCCAGGCGGTGATCGTCGGACTGTAGAAC200570.237371593353122No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGATCGTCGGACTGTA181990.21538244141364446No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC172620.2042931866411523No Hit
GGCCTCGATCCGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA141380.167321114165949No Hit
AATCCCCGATCCCCATCACGAATGGGGTTCAACGATCGTCGGACTGTAGA136810.16191258755866092No Hit
GTCTGTCAGGACCACGTCTTTCTTGTGATGATCGTCGGACTGTAGAACTC127150.15048012212618767Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAACT123760.14646810785951228No Hit
AATGTATTGAATCCATCATACGTCACAGAGATCGTCGGACTGTAGAACTC110390.13064491294935004Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGATCGTCGGA101060.11960299757823457No Hit
GGCCTCGATAAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA100640.11910593386377923No Hit
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTATAA100060.11841951254381705No Hit
CGTCACAGAATATTCACCAGCCTTTTCTGTGATCGTCGGACTGTAGAACT96100.11373291180752368No Hit
AGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA93270.11038364916012208No Hit
GTCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA92340.1092830080781138No Hit
GAAGCAGGTCGTCTACGAATGGTTTAGGATCGTCGGACTGTAGAACTCTG92020.1089042928671002Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCTCGATCAGAAGGACTTGGGCCCCCCAGATCGTCGGACTGTAGAACT89440.10585090147830298No Hit
CCTCGATCAGAAGGACTTGGGCCCCCCACGATCGTCGGACTGTAGAACTC87640.10372062841635145Illumina DpnII expression Sequencing Primer (100% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTCG2546050.043.6203771
TGCCTCG63750.041.106291
AAGCCTA45950.040.8794481
GTGCGAT79250.040.708011
AGAGTAC92100.039.9007873
GGCATCG63700.039.652041
GTACTAA94300.039.4098056
ACTAACC91000.039.1898238
TACTAAC95200.039.1302077
GAGTACT92750.038.9873734
CTATACG102400.038.83228716
CTTAGTA21050.038.7751548
AGTACTA95750.038.752765
CTCGATA73900.038.621164
CGATAAG68550.038.6047336
TATACGA103800.038.40861517
TTAGTAT22650.038.273299
TCGATAA72950.038.216415
GCAGAGT95900.038.2099271
ACTATAC104000.037.9904215
Produced by FastQC (version 0.11.2)