G124-G125-M20M38-NEBNext6_GCCAAT-_AC8KUAANXX_L008

Basic Statistics

Measure	Value
Filename	G124-G125-M20M38-NEBNext6_GCCAAT-_AC8KUAANXX_L008_001.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15946734
Sequences flagged as poor quality	0
Sequence length	50
%GC	51

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	949022	5.951199787994206	Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCC	67401	0.42266334912214626	No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC	39917	0.2503145785212194	No Hit
GGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCC	37611	0.23585393723881015	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG	35754	0.22420891951919433	Illumina Single End PCR Primer 1 (98% over 50bp)
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC	30835	0.19336247785910268	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGCCG	29533	0.18519779661465477	Illumina Single End PCR Primer 1 (98% over 50bp)
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC	22647	0.14201654081644555	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGGCG	20903	0.13108013214492698	Illumina Single End PCR Primer 1 (98% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	20108	0.12609478530211893	No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC	18792	0.11784231178622533	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	175895	43.772907	1
AGCGTCG	174445	43.71886	10
CGTCGTG	174720	43.69502	12
TCGTGTA	173355	43.690716	14
GAGCGTC	174935	43.626053	9
AGAGCGT	175645	43.60287	8
ATCGGAA	175725	43.598026	2
AAGAGCG	175035	43.548534	7
CGTGTAG	174795	43.53835	15
CGGAAGA	175260	43.478657	4
GTCGTGT	175725	43.47272	13
TCGGAAG	176810	43.45203	3
GATCTCG	129530	43.41339	34
GTAGGGA	175160	43.355766	18
ATCTCGG	130955	43.32881	35
TGTAGGG	177175	43.266285	17
TAGGGAA	175140	43.199657	19
GTAGATC	135850	43.16945	31
GTCGCCG	114905	43.147804	44
GAGTGTA	160385	43.122604	27