FastQCFastQC Report
Wed 23 Nov 2016
G124-G125-M20M38-NEBNext6_GCCAAT-_AC8KUAANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext6_GCCAAT-_AC8KUAANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15946734
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC15525799.736031214918366TruSeq Adapter, Index 6 (100% over 50bp)
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA1131260.7093991785402578No Hit
CCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCAC407250.25538144675894137No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA340070.21325369821808027No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC316210.19829138681312425No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG306280.19206440641701303No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT258760.16226520113773768No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG253000.15865317625540126No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA238990.14986767823430175No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGC236080.14804285316353807TruSeq Adapter, Index 6 (98% over 50bp)
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT230720.144681663342475No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGC223410.14009765259770435TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAGCTCGTATGC212690.13337527295557824TruSeq Adapter, Index 6 (98% over 50bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA195620.12267088671573753No Hit
GCGCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCT180710.11332100980677297No Hit
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC179640.11265002601786674No Hit
GGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGA160270.1005033381757042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1842900.043.90721
ATCGGAA1844850.043.7812542
TCGGAAG1854000.043.5853463
CGGAAGA1857650.043.4301344
ACGTCTG1856000.043.3716515
CGTCTGA1860950.043.36512416
GCACACG1873400.043.14106811
ACACGTC1878100.042.9678213
CACACGT1883800.042.89078512
GTCACGC1821800.042.87887629
AGTCACG1830050.042.86933528
TATCTCG1702650.042.86738239
CACGTCT1883600.042.83080314
TCACGCC1817750.042.81187430
CGCCAAT1801900.042.8097933
ACGCCAA1815550.042.661732
CACGCCA1817200.042.6360131
AGAGCAC1898900.042.608128
TCTGAAC1892150.042.5686218
GAACTCC1885900.042.5666921
Produced by FastQC (version 0.11.2)