G124-G125-M20M38-NEBNext5_ACAGTG-_AC8KUAANXX_L008

Basic Statistics

Measure	Value
Filename	G124-G125-M20M38-NEBNext5_ACAGTG-_AC8KUAANXX_L008_001.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17705690
Sequences flagged as poor quality	0
Sequence length	50
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	316753	1.7889898670992208	Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCC	110444	0.6237768762471273	No Hit
GGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCC	61355	0.34652702040982303	No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC	46386	0.26198357703088665	No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC	43107	0.24346410673630908	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	24561	0.1387181182998234	No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC	24535	0.13857127285070506	No Hit
GCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCC	22275	0.1258070145811883	No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC	19460	0.10990817076318403	No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG	18129	0.10239081334870316	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
AGCGTCG	58980	43.394756	10
TCGTGTA	58995	43.281796	14
CGTCGTG	59370	43.281387	12
CGTGTAG	59255	43.23652	15
GATCGGA	59765	43.19857	1
GAGCGTC	59270	43.17007	9
AAGAGCG	59380	42.908154	7
AGAGCGT	59865	42.855473	8
GATCTCG	44640	42.61662	34
GTCGTGT	60470	42.536324	13
ATCTCGG	45210	42.483566	35
ATCGGAA	60445	42.469276	2
GTCGCCG	39095	42.26879	44
TCGGAAG	60980	42.235004	3
GTAGGGA	60585	42.18032	18
CGGAAGA	60535	42.169964	4
TGTAGGG	61105	42.16161	17
GTAGATC	47245	41.9373	31
TGGTCGC	38540	41.714066	42
TAGGGAA	60975	41.656555	19