FastQCFastQC Report
Tue 26 Jan 2021
G124-G125-M20M38-NEBNext6_GCCAAT-_AC8KUAANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext6_GCCAAT-_AC8KUAANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15946734
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC15525799.736031214918366TruSeq Adapter, Index 6 (100% over 50bp)
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA1131260.7093991785402578No Hit
CCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCAC407250.25538144675894137No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA340070.21325369821808027No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC316210.19829138681312425No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG306280.19206440641701303No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT258760.16226520113773768No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG253000.15865317625540126No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA238990.14986767823430175No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGC236080.14804285316353807TruSeq Adapter, Index 6 (98% over 50bp)
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT230720.144681663342475No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGC223410.14009765259770435TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAGCTCGTATGC212690.13337527295557824TruSeq Adapter, Index 6 (98% over 50bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA195620.12267088671573753No Hit
GCGCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCT180710.11332100980677297No Hit
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC179640.11265002601786674No Hit
GGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGA160270.1005033381757042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)