FastQCFastQC Report
Tue 4 May 2021
TC4_F6_fPb_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_F6_fPb_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48959086
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG2137490.4365869902064757No Hit
AGCAGATTAAGAAACAAACGGCTCTTGCTGAGCTGGTGAAGCACAAGCCCAA857280.17510130805954996No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA840260.17162493597204817No Hit
GGATGACTTTGCACAGTTCCTGGATACATGTTGCAAGGCTGCTGACAAGGAC709460.1449087509517641No Hit
GTTGCAAGGCTGCTGACAAGGACACCTGCTTCTCGACTGAGGGTCCAAACCT596270.12178944680462375No Hit
CTTGTCACTAGATGCAAAGACGCCTTAGCCTAAACACATCACAACCACAACC552910.11293307232083541No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGT550190.11237750639380807No Hit
GCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTTTGGTGACTCT544810.11127862967049672No Hit
CTTCACCTTCCACTCTGATATCTGCACACTTCCAGAGAAGGAGAAGCAGATT501480.10242838275207997No Hit
CCTGCTTCTCGACTGAGGGTCCAAACCTTGTCACTAGATGCAAAGACGCCTT491280.10034501052572754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)