FastQCFastQC Report
Tue 4 May 2021
TC4_F5_fPb_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_F5_fPb_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37554282
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG1430570.3809339238598677No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA757760.20177725671868788No Hit
AGCAGATTAAGAAACAAACGGCTCTTGCTGAGCTGGTGAAGCACAAGCCCAA558240.14864882784871244No Hit
CTTGTCACTAGATGCAAAGACGCCTTAGCCTAAACACATCACAACCACAACC474510.12635310135872124No Hit
CCTGCTTCTCGACTGAGGGTCCAAACCTTGTCACTAGATGCAAAGACGCCTT448450.11941381278438501No Hit
CTTCACCTTCCACTCTGATATCTGCACACTTCCAGAGAAGGAGAAGCAGATT407750.10857616715984611No Hit
GGATGACTTTGCACAGTTCCTGGATACATGTTGCAAGGCTGCTGACAAGGAC398880.10621425274486675No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)